Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells.

The rate of glucose utilization by cells is known to be influenced by various environmental factors. The effects of hormones, such as insulin, and of drugs, such as 2,Pdinitrophenol, on carbohydrate metabolism have received considerable attention. A vast amount of literature has accumulated on the suppression of fermentation in the presence of air. However, information on the intracellular pacemakers of carbohydrate metabolism is rather scant. Some of the pertinent work on this problem will be reviewed, together with the Pasteur effect and the Crabtree effect, in the following paper (1). It is the purpose of this paper to report studies on the ratelimiting factors in glycolysis of Ehrlich ascites tumor cells. These cells were chosen because of their high rate of glycolysis and because of advantages in the use of cell suspensions for such studies. Moreover, preliminary studies on the mechanism of the Pasteur effect (2) in these cells made it desirable to obtain data on the rate-limiting factors under various experimental conditions.